Fig. 6

Volcano and box plot analyses of ocular lipid profiles of female Elovl4b KO and WT zebrafish normalized to tissue weight using a one-way ANOVA test. Volcano plot analysis showing two compounds significantly downregulated in female homozygous Elovl4b KO fish compared with WT age- and sex-matched controls (n = 3 each). Blue dots represent significantly downregulated lipid species; and gray dots represent lipid groups that were not significant in the comparison using our parameters. VLC-PUFA-containing species are circled in volcano plot comparisons. The box plots show the relative peak intensities of significantly altered lipids. Female WT (WT-F; teal green) and homozygous Elovl4b KO fish (KO-F; tan brown) (n = 3 each). The y-axes represent the relative peak intensities obtained through LC-MS/MS analysis. Significance was determined using the following parameters: P < 0.05, fold change >1.5, and false discovery rate adjusted.