Fig. 5

Volcano and box plot analyses of ocular lipid profiles of male Elovl4b KO and WT zebrafish normalized to tissue weight using a one-way ANOVA test. Volcano plot shows that seven compounds were significantly downregulated, whereas three compounds were significantly upregulated in ocular lipid comparisons between homozygous Elovl4b KO fish and WT age- and sex-matched controls (n ≥ 4 each). Blue dots represent significantly downregulated lipid species; red dots represent significantly upregulated lipid species; and gray dots represent lipid groups that were not significant in the comparison using our parameters. VLC-PUFA-containing species are circled in volcano plot comparisons. The box plots show the relative peak intensities of significantly altered lipids. Male WT (WT-M; orange) and homozygous Elovl4b KO fish (KO-M; dark purple) (n ≥ 4 each). The y-axes represent the relative peak intensities obtained through LC-MS/MS analysis. Significance was determined using the following parameters: P < 0.05, fold change >1.5, and false discovery rate adjusted.