FIGURE

Figure 4

ID

ZDB-FIG-240127-4

Publication

Anneser et al., 2023 - Molecular organization of neuronal cell types and neuromodulatory systems in the zebrafish telencephalon

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Figure 4

Glutamatergic cell types: molecular signatures and anatomical localization

(A) Correlation between the gene expression profile of individual cell types and the bulk transcriptome of pallial regions. The analysis included 734 genes that were differentially expressed between brain regions in the bulk sequencing dataset and among the 2,000 most variable genes of the scRNA-seq dataset.

(B) Hierarchical molecular signature distinguishing glutamatergic cell types in the dorsal pallium.

(C–F) Maximum intensity projection of gene expression patterns along the dorsoventral axis obtained from hybridization chain reaction (HCR) against two exemplary marker genes per brain region (cyan; z-projection of confocal images) overlaid on the snap25a registration channel (gray). Position of cross-section is indicated by dotted line. Left: location of the four pallial brain areas in a dorsal view and coronal sections of the adult zebrafish brain. Scale bars, approximately 200 μm (not precise due to registration of image datasets onto the reference brain).

Related to Figures S4 and S7 and Data S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.