Fig. 4

Individual bacterial members of zebrafish microbiota exhibit differential effects on HSPC development (A) Quantification of cmyb staining in WT CONR, GF larvae, and GF larvae at 3 dpf mono-associated with a specific bacterial strain. Bacterial mono-associations are labeled by genus. Different bacterial species within the same genus are labeled with a number (1, 2, 3, and so on). (B) WISH for cmyb in WT CONR, GF larvae, and GF larvae treated with bacterial strains Aero. 1 or Aero. 10. (C) Quantification of WISH results from (B). (D) qPCR analysis of runx1 and cmyb expression in the kidney at 7 dpf of WT CONR, GF larvae, and GF larvae treated with Aero. 1 or Aero. 10. (E) WISH for mpx in WT CONR, GF larvae, and GF larvae treated with Aero. 1 or Aero. 10. (F) Quantification of WISH results from (E). (G) qPCR analysis of mpx expression at 7 dpf in whole embryos of WT CONR, GF larvae, and GF larvae treated with Aero. 1 or Aero. 10. ANOVA in all analyses; mean ± SEM of three independent experiments, n = 45–60 larvae per group for WISH, 30–40 larvae per group for qPCR analysis, ∗p < 0.05, ∗∗p < 0.01, ns, not significant. Scale bars, 200 μm. See also Figure S4.