PUBLICATION
The microbiota regulates hematopoietic stem and progenitor cell development by mediating inflammatory signals in the niche
- Authors
- Zhong, D., Jiang, H., Zhou, C., Ahmed, A., Li, H., Wei, X., Lian, Q., Tastemel, M., Xin, H., Ge, M., Zhang, C., Jing, L.
- ID
- ZDB-PUB-230217-35
- Date
- 2023
- Source
- Cell Reports 42: 112116112116 (Journal)
- Registered Authors
- Jing, Lili
- Keywords
- CP: Microbiology, CP: Stem cell research, chd8, commensal microbiota, hematopoietic stem and progenitor cell development, inflammatory signals, zebrafish
- MeSH Terms
-
- Animals
- Bone Marrow
- Cytokines/metabolism
- Hematopoiesis
- Hematopoietic Stem Cells*/metabolism
- Stem Cell Niche
- Zebrafish*
- PubMed
- 36795566 Full text @ Cell Rep.
Citation
Zhong, D., Jiang, H., Zhou, C., Ahmed, A., Li, H., Wei, X., Lian, Q., Tastemel, M., Xin, H., Ge, M., Zhang, C., Jing, L. (2023) The microbiota regulates hematopoietic stem and progenitor cell development by mediating inflammatory signals in the niche. Cell Reports. 42:112116112116.
Abstract
The commensal microbiota regulates the self-renewal and differentiation of hematopoietic stem and progenitor cells (HSPCs) in bone marrow. Whether and how the microbiota influences HSPC development during embryogenesis is unclear. Using gnotobiotic zebrafish, we show that the microbiota is necessary for HSPC development and differentiation. Individual bacterial strains differentially affect HSPC formation, independent of their effects on myeloid cells. Early-life dysbiosis in chd8-/- zebrafish impairs HSPC development. Wild-type microbiota promote HSPC development by controlling basal inflammatory cytokine expression in kidney niche, and chd8-/- commensals elicit elevated inflammatory cytokines that reduce HSPCs and enhance myeloid differentiation. We identify an Aeromonas veronii strain with immuno-modulatory activities that fails to induce HSPC development in wild-type fish but selectively inhibits kidney cytokine expression and rebalances HSPC development in chd8-/- zebrafish. Our studies highlight the important roles of a balanced microbiome during early HSPC development that ensure proper establishment of lineal precursor for adult hematopoietic system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping