FIGURE

Fig. 7

ID

ZDB-FIG-231215-62

Publication

Kawaguchi et al., 2019 - Co-option of the PRDM14-CBFA2T complex from motor neurons to pluripotent cells during vertebrate evolution

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Fig. 7

The pre-PR domain is responsible for the incompatibility of sPRDM14 with mCBFA2T2. (A) Electrostatic charge distribution of the binding pocket of mPRDM14 and sPRDM14 with mCBFA2T2. (B) Residues involved in the negative charge of the binding surface of sPRDM14 and mPRDM14 with mCBFA2T2 are shown as sticks. (C) Alignment of the amino acid sequences of the pre-PR and PR domain derived from mPRDM14, zPRDM14, amPDM14 and sPRDM14. Red asterisks indicate the amino acids involved in hydrogen bonding with mCBFA2T2. (D) Electrostatic charge distribution of the binding pocket of chimeric protein constructed by sPRDM14 and mPRDM14. (E) Western blot analysis of sPRDM14, s/mPRDM14, m/sPRDM14 and mPRDM14. (F) Colonies stained for AP activity in Prdm14 KO ESCs expressing the empty vector, sPRDM14, s/mPRDM14, m/sPRDM14 or mPRDM14, after being transferred to medium containing serum plus LIF for 9 days. Scale bar: 50 μm. (G) Co-IP of sPRDM14, s/mPRDM14, m/sPRDM14 or mPRDM14 with mCBFA2T2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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