FIGURE

Figure 5

ID

ZDB-FIG-231023-9

Publication

Deng et al., 2023 - Ypel5 regulates liver development and function in zebrafish

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Figure 5

PPARα signaling mediates the regulation of Hnf4a by Ypel5. (A) GSEA showing the enrichment of the PPAR signaling pathway in ypel5^−/− mutants. (B) Genomic browser view showing ChIP–seq signals of PPARα (GSM864671, GSM1163175) and H3K27ac (GSM2540257) at the Hnf4a gene locus in hepatocytes. The peaks are highlighted in yellow. (C) Luciferase activity assay in Huh-7 cells transfected with control vectors or PPARα and reporter constructs. The Renilla plasmid was used as an internal control. (D) Western blot analysis of Huh-7 cells treated for 48 h with control vehicle or GW6471. (E) qPCR analysis of hnf4a expression between control siblings and ypel5^−/− mutants treated for 48 h with bezafibrate. Data shown are mean ± SEM. Student's t-test. n.s., not significant; ***P < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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