IMAGE

Figure 5

ID

ZDB-IMAGE-231023-8

Genes

hnf4a

Publication

Deng et al., 2023 - Ypel5 regulates liver development and function in zebrafish

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Figures for Deng et al., 2023

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Figure 5

PPARα signaling mediates the regulation of Hnf4a by Ypel5. (A) GSEA showing the enrichment of the PPAR signaling pathway in ypel5^−/− mutants. (B) Genomic browser view showing ChIP–seq signals of PPARα (GSM864671, GSM1163175) and H3K27ac (GSM2540257) at the Hnf4a gene locus in hepatocytes. The peaks are highlighted in yellow. (C) Luciferase activity assay in Huh-7 cells transfected with control vectors or PPARα and reporter constructs. The Renilla plasmid was used as an internal control. (D) Western blot analysis of Huh-7 cells treated for 48 h with control vehicle or GW6471. (E) qPCR analysis of hnf4a expression between control siblings and ypel5^−/− mutants treated for 48 h with bezafibrate. Data shown are mean ± SEM. Student's t-test. n.s., not significant; ***P < 0.001.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.