FIGURE

Figure 1.

ID

ZDB-FIG-231012-2

Publication

Oyarbide et al., 2023 - SBDS^R126T rescues survival of sbds^-/- zebrafish in a dose-dependent manner independently of Tp53

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Figure 1.

Generation and characterization of the zebrafish transgenic strain expressing SBDS^R126T.

(A) Constructs to create the transgenic line expressing zebrafish sbds and human SBDS^R126T were injected into fertilized eggs and, subsequently, strains were inbred. (B) Western blotting showed Sbds/SBDS^R126T expression in zebrafish fins on 1-yr-old fish. (C) The number of transgene copies was determined in the zebrafish SBDS^R126T line. (D) mRNA levels of human SBDS in 2 dpf larvae with one and two copies of the transgene Tg(SBDS^R126T). (E) Immunoblot showing protein levels in fish with one and two copies of the transgene comparing with WT sbds. ubi-ubiquitin promoter, cmlc2 green heart marker to aid screening of transgenesis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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