FIGURE

Figure 2.

ID

ZDB-FIG-231012-3

Publication

Oyarbide et al., 2023 - SBDS^R126T rescues survival of sbds^-/- zebrafish in a dose-dependent manner independently of Tp53

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Figure 2.

Phenotype and biochemical analysis of adult zebrafish lines expressing the human SBDS^R126T.

(A) Zebrafish cross between sbds heterozygous and transgenic line. (B) Standard length in 1-yr-old fish. (C) H&E staining of internal organs of 1-yr-old male fish. (D) Flow cytometric analysis of whole kidney marrows showing the different blood cell types in the presence or absence of the transgene SBDS^R126T, in the different zebrafish sbds backgrounds. (E) Polysome profile of the liver. (F) Western blotting demonstrated Eif6 accumulation in zebrafish sbds KO with the transgene. (G, H) Protein quantification (H) qRT-PCR analysis of liver, brain, and GI showing up-regulation of cdkn1a in all tissues. AUFS, absorbance units full scale.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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