ZFIN Image: Oyarbide et al., 2023, Figure 1.

Image

Figure Caption

Figure 1. Generation and characterization of the zebrafish transgenic strain expressing SBDS^R126T.

(A) Constructs to create the transgenic line expressing zebrafish sbds and human SBDS^R126T were injected into fertilized eggs and, subsequently, strains were inbred. (B) Western blotting showed Sbds/SBDS^R126T expression in zebrafish fins on 1-yr-old fish. (C) The number of transgene copies was determined in the zebrafish SBDS^R126T line. (D) mRNA levels of human SBDS in 2 dpf larvae with one and two copies of the transgene Tg(SBDS^R126T). (E) Immunoblot showing protein levels in fish with one and two copies of the transgene comparing with WT sbds. ubi-ubiquitin promoter, cmlc2 green heart marker to aid screening of transgenesis.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Life Sci Alliance