corastl325 is a null allele of taf5. (A) Brightfield images of control, corastl325, taf5stl852, and corastl325/ taf5stl852 embryos at 96 hpf. (B) Measurement of pericardial area and ejection fraction in control, corastl325, taf5stl852, and corastl325/ taf5stl852 embryos at 96 hpf. n=22. (C) Measurement of head-to-body and head-to-eye ratios in control, corastl325, taf5stl852, and corastl325/ taf5stl852 embryos at 96 hpf. n=22. (D) Survival of control, taf5stl852, and corastl325/taf5 CRISPR embryos. n=42. (E) RTPCR for taf5 mRNA in control, corastl325, taf5stl852, and corastl325/ taf5stl852 embryos at 96 hpf. n=7. (F) Time course of taf5 mRNA expression by whole-mount in situ hybridization (top) and taf5 mRNA expression in corastl325 and taf5stl852 embryos at 96 hpf. Note: all brackets marked with ‘*’ represent P<1E-4.
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