FIGURE

Fig. 4

ID

ZDB-FIG-231002-47

Publication

Yao et al., 2023 - The Roles of Histamine Receptor 1 (hrh1) in Neurotransmitter System Regulation, Behavior, and Neurogenesis in Zebrafish

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Fig. 4

Proliferation and differentiation markers in hrh1^−/− zebrafish. a Representative pictures (ventral view) for immunohistochemistry of HuC/D in hrh1^+/+ and hrh1^−/− larval brain at 6 dpf. Anterior to the left. Scale bar: 100 µm. N = 6. c Representative pictures (dorsal view) for immunohistochemistry of PCNA in hrh1^+/+ and hrh1^−/− larval brain at 6 dpf. Anterior to the left. Scale bar: 100 µm. N = 6. i Representative pictures (ventral view) for in situ hybridization of pax2a and pax6a in hrh1^+/+ and hrh1^−/− larval brain at 6 dpf. Anterior to the left. Scale bar: 100 µm. b, d–h qPCR results of elavl3, pcna, olig2, slc1a3a, gfap, and isl1 in whole larval zebrafish at 6 dpf. N (hrh1^+/+) = 5, N (hrh1^−/.⁻) = 6. All histograms are exhibit as mean ± SEM, unpaired t test, two tailed. **0.001 < P ≤ 0.01

Expression Data

Genes:	elavl3 gfap isl1a olig2 pax2a pax6a pcna slc1a3a
Antibodies:	Ab1-elavl Ab2-pcna
Fish:	WT hrh1^{zf4150/zf4150}
Anatomical Terms:	brain brain neuron whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hrh1^{zf4150/zf4150}
Observed In:	whole organism
Stage:	Day 6

Phenotype Detail

Acknowledgments

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