Fluorescence in situ hybridization confirms minimal co-localization of gsx1 and gsx2 during embryonic development. (Ai-Avi) Lateral views of gsx1 and gsx2 expression at 24 hpf. (Bi-Biii) Ventral view of gsx1 and gsx2 expression at 24 hpf. (Biv-Bvi) Cross-sectional view taken at the dashed line in Avi. (Ci-Cvi) Lateral views of gsx1 and gsx2 expression at 30 hpf. (Di-Diii) Ventral view of gsx1 and gsx2 expression at 30 hpf. (Div-Dvi) Cross-sectional view taken at the dashed line in Cvi. Lateral views were taken at ×20 and ventral/Cross-sectional views were taken at ×40, all with anterior facing left. All were pseudocolored using FIJI ImageJ and scale bars represent 100 μm. For lateral views, eyes were dissected off. Main images are max z-projections and insets are single z-stack slices zoomed into the boxed region shown in the main image. Di, diencephalon; Hb, hindbrain; Mb, midbrain; Sc, spinal cord; Tel, telencephalon
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