FIGURE

FIGURE 3

ID

ZDB-FIG-230724-42

Publication

Chi et al., 2023 - The interplay of common genetic variants NRG1 rs2439302 and RET rs2435357 increases the risk of developing Hirschsprung's disease

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FIGURE 3

Phenotypes of nrg1 and ret knockdown zebrafish. (A) qRT-PCR analysis of relative expression levels of nrg1 and ret in wildtype, nrg1^−/−, ret^−/−, and nrg1^−/−ret^−/− zebrafish embryos at 72 hpf. (B) The survival and deformity rate of wildtype, nrg1^−/−, ret^−/−, and nrg1^−/−ret^−/− zebrafish at 72 hpf. (C) Whole-mount in situ hybridization in wildtype, nrg1^−/−, ret^−/−, and nrg1^−/−ret^−/− embryos at 72 hpf for nrg1, ret, and sox10. White arrows indicated the end of the positive expression of each gene. (D) Immunofluorescence staining and quantitative analysis of a neuronal marker HuC/D in wildtype, nrg1^−/−, ret^−/−, and nrg1^−/−ret^−/− embryos at 72 hpf.

Expression Data

Genes:	nrg1 ret sox10
Antibody:	Ab5-elavl
Fish:	WT nrg1^{ihb534/ihb534} ret^{ihb309/ihb309} ret^{ihb309/ihb309}; nrg1^{ihb534/ihb534}
Anatomical Terms:	embryonic structure enteric neuron whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nrg1^{ihb534/ihb534} ret^{ihb309/ihb309} ret^{ihb309/ihb309}; nrg1^{ihb534/ihb534}
Observed In:	embryonic structure enteric neuron pericardium whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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