FIGURE

FIGURE 5

ID

ZDB-FIG-230724-32

Publication

Chi et al., 2023 - The interplay of common genetic variants NRG1 rs2439302 and RET rs2435357 increases the risk of developing Hirschsprung's disease

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FIGURE 5

Differentiation ability of SHSY-5Y cells and IPSCs with different genotype. (A) Differentiation of SHSY-5Y cells were induced by ATRA and ENCCs were obtained from IPSCs by adding LDN193189 and SB431542 to the differentiation medium. (B) Immunofluorescence staining of a SHSY-5Y differentiation marker NSE and bright field morphology of SHSY-5Y cells with different genotypes. (C) Semi-quantitative analysis of a SHSY-5Y differentiation marker NSE in SHSY-5Y cells with different genotypes. (D) Flow cytometry analysis of the ENCC differentiation rate (HNK-1+ p75NTR+) of IPSCs with different genotypes. (E) Luciferase assay showed that CTCF had significantly higher binding ability to SNP rs2439302 CC than GG in SHSY-5Y cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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