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FIGURE 3
Phenotypes of nrg1 and ret knockdown zebrafish. (A) qRT-PCR analysis of relative expression levels of nrg1 and ret in wildtype, nrg1−/−, ret−/−, and nrg1−/−ret−/− zebrafish embryos at 72 hpf. (B) The survival and deformity rate of wildtype, nrg1−/−, ret−/−, and nrg1−/−ret−/− zebrafish at 72 hpf. (C) Whole-mount in situ hybridization in wildtype, nrg1−/−, ret−/−, and nrg1−/−ret−/− embryos at 72 hpf for nrg1, ret, and sox10. White arrows indicated the end of the positive expression of each gene. (D) Immunofluorescence staining and quantitative analysis of a neuronal marker HuC/D in wildtype, nrg1−/−, ret−/−, and nrg1−/−ret−/− embryos at 72 hpf.