Figure 1

Identification of cilium-associated genes with enriched expression in zebrafish hair cells. (A) Diagram of a generic sensory hair cell. (B) Scatter plot of differentially expressed genes identified by TU-tagging. ankef1a, odf3l2a, and saxo2 are indicated in the 4TU-UPRT-enriched population. (C–E) mRNA in situ hybridization (ISH) for ankef1a, odf3l2a, and saxo2 in zebrafish larvae at 3 days post-fertilization (dpf). NMs = neuromasts; OE = olfactory epithelium. In the ankef1a and saxo2 posterior macula panels, arrows point to the posterior macula sensory patch and asterisks indicate the out-of-focus anterior macula. (F) Schematic of transgenic constructs used in this study. (G) Overview of Ankef1a-GFP (1), Odf3l2a-GFP (2), Saxo2-GFP (3), Saxo2-mKate2 (4), YFP-TUBA (5), and Actb1-GFP (6) protein localization in the lateral cristae of larval zebrafish at 5–6 dpf. Scale bars are 100 µm for the headshots in (C–E), and 20 µm for all other ISH panels. Scale bars are 5 µm in (G).