FIGURE

Figure 5

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ZDB-FIG-230621-50

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Ochi et al., 2020 - CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions

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Figure 5

Chlamydomonas VFL3 Protein Localizes to Basal Bodies and the Proximal Ends of Flagella

(A) Rescue of abnormal flagella numbers in vfl3 strains by wild-type VFL3. Bar chart showing flagella numbers observed in wild-type strains (CC-124 and CC125), mutant strains (vfl3-1 and vfl3-2), and the vfl3-1 and vfl3-2 strains expressing VFL3 constructs in Chlamydomonas. The numbers of cells “n” used for calculating ratio flagella numbers are shown on the right side of the chart. A χ² test was used to determine if the number of cells with zero flagella was significantly different. NS, not significant; ^∗∗∗p < 0.0001.

(B) Wild-type VFL3 protein localizes to Chlamydomonas basal bodies. In the first column, cells were stained with acetylated α-tubulin (red) for cilia and rootlet microtubules, anti-HA (green) for UNI2, and anti-GFP (magenta) for VFL3. Scale bar, 4 μm. Magnified views (4×) of the basal body regions (white boxes) are shown on the other three columns. Scale bars, 1 μm.

(C) Localization of VFL3 is affected in the 5E mutant. In the first column, cells were stained with acetylated α-tubulin (red) for cilia and rootlet microtubules, anti-HA (green) for wild-type and mutant VFL3, and anti-BLD10/CEP135 (magenta). Scale bar, 4 μm. Magnified views (4×) of the basal body regions (white boxes) are shown on the other three columns. Scale bar, 1 μm.

See also Figure S6.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Structure (London, England : 1993), 28, Ochi, T., Quarantotti, V., Lin, H., Jullien, J., Rosa E Silva, I., Boselli, F., Barnabas, D.D., Johnson, C.M., McLaughlin, S.H., Freund, S.M.V., Blackford, A.N., Kimata, Y., Goldstein, R.E., Jackson, S.P., Blundell, T.L., Dutcher, S.K., Gergely, F., van Breugel, M., CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions, 674689.e11674-689.e11, Copyright (2020) with permission from Elsevier. Full text @ Structure