flrt2 is expressed in developing zebrafish eyes. (A,D″) Lateral views of flrt2 mRNA whole-mount in situ hybridization of (A,A′) 24 hpf, (B,B′) 36 hpf, (C,C′) 48 hpf, and (D,D′) 72 hpf zebrafish embryos. (A″-D″) Dorsal views of flrt2 mRNA whole-mount in situ hybridization of (A″) 24 hpf, (B″) 36 hpf, (C″) 48 hpf, and (D″) 72 hpf zebrafish embryos. (E-H″) Embryos exposed to a sense riboprobe were used as a negative control. n=20/each group. Scale bar: 200 µm. Leica stereomicroscope M205 was used: objective: M205FA 30×/161×; scaling (per pixel): 1.00 pixel×1.00 pixel; image size (pixels): 1392×1040.
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