FIGURE

Fig 8

ID

ZDB-FIG-230621-17

Publication

Guan et al., 2016 - Phosphorylation of Def Regulates Nucleolar p53 Turnover and Cell Cycle Progression through Def Recruitment of Calpain3

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Fig 8

Def phosphorylation modulates its role in cell cycle in zebrafish and knockdown of CAPN3 or Def in cultured human cells causes a p53-dpendent cell cycle arrest.

(A) Histogram showing flow cytometry analysis of the ratio of G1, S, and G2/M phases of liver cells collected from 8-dpf zebrafish of different genotypes as shown. (B) Western blot showing the effect of CAPN3 or hu-Def knockdown on p53 protein level in HCT116-p53^+/+ or HCT116-p53^-/- cells at 24 h post treatment. β-actin: loading control. (C) Histogram showing flow cytometry analysis of the ratio of cells at G1, S, and G2/M phases in HCT116-p53^+/+ (left panel) and HCT116-p53^-/- cells (right panel) treated with ctrl-siRNA, capn3-siRNA, or hu-def-siRNA at 24 h post treatment. Data are presented as means with SD. Columns with no common letter are significantly different (p < 0.05). Underlying data for A and C are provided in S1 Data.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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