FIGURE

Fig 5

ID

ZDB-FIG-230621-14

Publication

Guan et al., 2016 - Phosphorylation of Def Regulates Nucleolar p53 Turnover and Cell Cycle Progression through Def Recruitment of Calpain3

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Fig 5

S50, S58, S62, S87, and S92 are modified by phosphorylation.

(A–E) Western blot (using an anti-EGFP antibody) of EGFP-D14 and its various single mutant (A, B), or EGFP-D14 and various double mutant protein derived from EGFP-D14_S50A (C), or EGFP-D14_S50,58,62A triple mutant protein (D), or EGFP-D14_S50,58,62,87,92A penta mutant protein (E) to analyse the gel mobility shift of these EGFP-D14 derivatives treated with (+) or without (-) CIP as shown. (F) Mass spectrum analysis of the EGFP-D14 peptide expressed by 293T cells. S50, S58, and S62 (upper panel), S87 and S92 (lower panel) five serine residues (pS) were identified to be modified by phosphorylation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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