FIGURE

Fig 7

ID

ZDB-FIG-230621-16

Publication

Guan et al., 2016 - Phosphorylation of Def Regulates Nucleolar p53 Turnover and Cell Cycle Progression through Def Recruitment of Calpain3

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Fig 7

Simultaneous phosphorylations at S58 and S62 or at S87 and S92 are necessary for Def to promote cell cycle progression.

(A, B) Representative images of P-H3, EdU, and PCNA immunostaining in def^-/-Tg(LF:S58,62A)-6 or -13 two lines and relavant control lines (A). Scale bar: 100 μm. Hepatocytes were marked by showing DsRed fluorescence produced by the Tg(fabp10a:dsRed; elastase:GFP) transgenic fish (images for P-H3 and Edu staining) or by an Fabp10a antibody (images for PCNA staining). Nuclei were stained by DAPI. Statistical data for the ratio of P-H3, EdU, and PCNA positive cells in each genotype at 2.5 and 3 dpf were shown, respectively. (B) Data are presented as means with SD. Columns with no common letter are significantly different (p < 0.05, one-way ANOVA with Tukey’s post hoc test). (C) Histogram showing the statistical data for the ratio of P-H3 positive cells in def^-/-Tg(LF:S87,92A)-1 or -2 two lines and relavant control lines. Underlying data for B and C are provided in S1 Data.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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