Fig 2
- ID
- ZDB-FIG-230423-2
- Publication
- Bolten et al., 2023 - Zebrafish (Danio rerio) larvae as a predictive model to study gentamicin-induced structural alterations of the kidney
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Gentamicin-induced damage to the pronephros in 96 hpf Tg(wt1b:eGFP) ZFL.
(A) Schematic representation of renal pronephros in 96 hpf ZFL. GL: glomerulus, PT: proximal convoluted and straight tubule, DT: distal early and late tubule. Pronephros are fused together at the midline above the yolk and heart. Transgenic expression of eGFP leads to a fluorescent signal (green) in the glomerulus and upper parts of the tubules. (B) 72 hpf Tg(wt1b:eGFP) ZFL were injected with 1 nL of 14.4 mM to 42 mM gentamicin. Representative images of maximum projections. The color-coded heat map indicates SI, e.g. white correlates to high SI values and black to weak SI. Scale bar: 25 ?m. Gent: gentamicin. (C) Relative SI and relative volume of the glomerulus (panel B, white dotted rectangle) as compared to normalized control is shown using a box-and-whisker plot, n ? 10 ZFL. *p < 0.001, **p < 0.05. |