ZFIN Image: Bolten et al., 2023, Fig 2

Image

Figure Caption

Fig 2

Gentamicin-induced damage to the pronephros in 96 hpf Tg(wt1b:eGFP) ZFL.

(A) Schematic representation of renal pronephros in 96 hpf ZFL. GL: glomerulus, PT: proximal convoluted and straight tubule, DT: distal early and late tubule. Pronephros are fused together at the midline above the yolk and heart. Transgenic expression of eGFP leads to a fluorescent signal (green) in the glomerulus and upper parts of the tubules. (B) 72 hpf Tg(wt1b:eGFP) ZFL were injected with 1 nL of 14.4 mM to 42 mM gentamicin. Representative images of maximum projections. The color-coded heat map indicates SI, e.g. white correlates to high SI values and black to weak SI. Scale bar: 25 ?m. Gent: gentamicin. (C) Relative SI and relative volume of the glomerulus (panel B, white dotted rectangle) as compared to normalized control is shown using a box-and-whisker plot, n ? 10 ZFL. *p < 0.001, **p < 0.05.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One