FIGURE

FIGURE 5

ID

ZDB-FIG-230307-24

Publication

Kim et al., 2023 - EWSR1 prevents the induction of aneuploidy through direct regulation of Aurora B

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 5

The EWSR1 knockdown cells do not arrest at any mitotic stages. (A). The percentages of mitotic stage of AUX- and AUX + cells released for 0, 30, 60 and 90 min after the thymidine/nocodazole treatment (total of 83–173 cells per sample, n = 3 experiments). NS = non-significant. (B). Representative images of western blotting using anti-FLAG (top panel), anti-Cyclin B (middle panel) and anti-β actin (bottom panel) obtained from AUX- and AUX + cells released at 0, 30, 60, and 90 min after the thymidine/nocodazole treatment. *: Non-specific band. (C). Quantification of the levels of Cyclin B protein (normalized by β-actin) obtained from AUX- and AUX + cells (n = 3 experiments). Images of western blotting gel using anti-EWSR1 (top panel), anti-Cyclin B (middle panel), and anti-β-actin antibodies (bottom panel). Values are mean with S.D. using two-way ANOVA with Tukey’s multiple comparison test. NS: Non-Significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol