FIGURE

FIGURE 6

ID

ZDB-FIG-230307-25

Publication

Kim et al., 2023 - EWSR1 prevents the induction of aneuploidy through direct regulation of Aurora B

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 6

The EWSR1 knockdown promotes the induction of aneuploidy. (A). Schematic diagram for the mitotic synchronization of AID-EWSR1 knockdown (synchronized to mitosis using thymidine/nocodazole, and treated with AUX for 48 h concurrently) cells. The mitotic cells were harvested 30 min after released from the arrest. (B). Representative images of chromosomes visualized with anti-CENP-C (red), and anti-Topoisomerase II (green) obtained from AUX- and AUX + cells. Scale bar = 10 um. (C). The percentage of cells that displayed normal 46 numbers of chromosomes was decreased in AUX + cells compared to AUX-cells. 18-24 cells per sample were counted from n = 3 experiments (Total of n = 56 cells from AUX-, and of n = 62 cells from AUX+). Two-tailed paired t-test, *p < 0.05. (D). The percentage of of cells with each of the chromosome numbers in AUX− and AUX + cells n = 3 experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol