FIGURE 5

The EWSR1 knockdown cells do not arrest at any mitotic stages. (A). The percentages of mitotic stage of AUX- and AUX + cells released for 0, 30, 60 and 90 min after the thymidine/nocodazole treatment (total of 83–173 cells per sample, n = 3 experiments). NS = non-significant. (B). Representative images of western blotting using anti-FLAG (top panel), anti-Cyclin B (middle panel) and anti-β actin (bottom panel) obtained from AUX- and AUX + cells released at 0, 30, 60, and 90 min after the thymidine/nocodazole treatment. *: Non-specific band. (C). Quantification of the levels of Cyclin B protein (normalized by β-actin) obtained from AUX- and AUX + cells (n = 3 experiments). Images of western blotting gel using anti-EWSR1 (top panel), anti-Cyclin B (middle panel), and anti-β-actin antibodies (bottom panel). Values are mean with S.D. using two-way ANOVA with Tukey’s multiple comparison test. NS: Non-Significant.