FIGURE

Figure 2

ID
ZDB-FIG-230220-2
Publication
Grepper et al., 2023 - Methodological advancements in organ-specific ectopic lipid quantitative characterization: Effects of high fat diet on muscle and liver intracellular lipids
Other Figures
All Figure Page
Back to All Figure Page
Figure 2

Study design and high fat diets validation. (A) Experimental design. Three cohorts of 50 larvae per group were studied at 15, 18 and 21 dpf. (B) Survival curve. Bars represent mean ± SEM. Starting n = 250 in three independent cohorts (3 × 3 ANOVA main effect of diet (F(2, 111) = 0.499, p = 0.609)). (C) Larvae length. Whiskers represent min. to max., n = 36 pooled from three independent cohorts (3 × 3 ANOVA main effect of diet (F(2, 317) = 1.628, p = 0.198)). (D) Representative Oil red O (ORO) images of 21 dpf larvae. (E) ORO extraction absorbance at 495 nm normalized to unstained samples. Whiskers represent min. to max., n = 36 per diet for each of the three independent experiments. (F) Representative Nile Red images of visceral adipocytes at 21 dpf. (G) Adipocyte count distribution per diet. Median and 75th quartile are shown by white lines, n = 33 per diet (Kruskal–Wallis test with Dunn’s multiple comparisons correction). For all panels: #p = 0.0525, ∗p < 0.05, ∗∗p ≤ 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol Metab