FIGURE

Figure 1

ID

ZDB-FIG-230220-1

Publication

Grepper et al., 2023 - Methodological advancements in organ-specific ectopic lipid quantitative characterization: Effects of high fat diet on muscle and liver intracellular lipids

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Figure 1

Stepwise larvae embedding, cryopreservation and cryosectioning. (A) Custom mold used to form imprints in embedding medium and red waxed dental floss used to mark region of interest (black arrowhead). For exact dimensions, see supplemental data. (B) Fixed whole larvae are aligned in imprints. (C) After covering with embedding medium, blocks are shaped and cryoprotected with sucrose. (D) Blocks are frozen in liquid nitrogen and cut with the cryostat. (E) Representative sagittal section of 21 dpf larva fed with normal diet (14% fat diet). (F) Close view of the abdomen with muscle (pink dashed line) and liver landmarks (blue dashed line). B: Brain, E: Esophagus, G: Gut, H: Heart, L: Liver, SB: Swim Bladder, SM: Skeletal Muscle. Actin labeled with phalloidin (red), cell membrane labeled with WGA (green), LDs stained with Bodipy665 (white), nuclei stained with Hoechst (blue).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol Metab