Figure 3

Effects of embryonic ethanol (0.5% v/v, 22?24 hpf) and Cxcr4 antagonist AMD3100 (1 µM, 2?24 hpf) on locomotor and anxiety-like behaviors in 8 dpf zebrafish. (a) Bar graphs (n = 29/group) show that ethanol compared to control increases locomotor activity, as indicated by an increase in distance traveled, and this ethanol effect is blocked by pretreatment with AMD3100. (b) Bar graphs (n = 29/group) show that ethanol increases anxiety-like behavior, indicated by an increase in perecentage of time spent in the perimeter of the arena (thigmotaxis), and this behavioral effect is blocked by AMD3100. (c) Bar graphs (n = 25/group) show that ethanol increases another anxiety-like behavior, indicated by an increase in percentage of time spent in the light half of the arena during the light?dark test (light preference), and this behavioral effect is blocked by AMD3100. *p < 0.05. Results are shown as means ± standard errors. hpf hours post fertilization, dpf days post fertilization.