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Figure 2

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ZDB-FIG-230131-55

Publication

Collier et al., 2023 - Neuronal chemokine concentration gradients mediate effects of embryonic ethanol exposure on ectopic hypocretin/orexin neurons and behavior in zebrafish

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Figure 2

Effects of exposure to embryonic ethanol (0.5% v/v, 22?24 hpf) and Cxcr4 antagonist AMD3100 (1 �M, 2?24 hpf) on the location of Hcrt neurons in the aAH, pAH and POA of 6 dpf zebrafish brains. (a) Photomicrographs (�25, overlay of n = 4 images in each condition) illustrate zebrafish brains, in a dorsal/ventral view obtained at 6 dpf using live-imaging, with pan-neuronal vglut2a expression (grey) and also Hcrt neurons (green) normally located in the aAH and pAH of control, ethanol, and ethanol + AMD3100 zebrafish and ectopically located in the POA of only ethanol zebrafish. (b) Bar graphs (n = 4/group) show that ethanol compared to control increases the number of Hcrt neurons in the aAH and induces ectopic neurons in the POA while having no effect in the pAH, and these effects on Hcrt neurons in the aAH and POA are totally blocked by AMD3100. Scale bar 20 �m. *p < 0.05 compared to ethanol. Results are shown as means � standard errors. aAH anterior part of the anterior hypothalamus, pAH posterior part of the anterior hypothalamus, POA preoptic area, Hcrt hypocretin, hpf hours post fertilization, dpf days post fertilization.

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