Fig. 5
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- ZDB-FIG-230129-5
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- Wu et al., 2021 - Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale
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Figure 5. Migrasome dynamics during neutrophil migration in mouse liver (A) MIPs with magnified views illustrating the formation of migrasomes during neutrophil (yellow, Ly-6G/Ly-6C) migration along vessels (magenta, WGA; Video S3, part I). (B) Comparisons between two-photon microscopy, SDCM, and DAOSLIMIT. (C) Migrasome formation with gradually increasing fluorescence (Video S3, part II). (D) Average temporal trace of the newly generated migrasome marked with the red box in (C). (E) 3D migration of one migrasome in a vessel with a temporal-coded trace (Video S4, part I). (F) One migrasome remained attached to the vessel (Video S4, part II). (G) The migrasome generated by one neutrophil was picked up by another neutrophil (Video S4, part III). (H) Division process of a migrasome attached on the vessel (Video S4, part IV). Scale bar, 10 ?m. See also Videos S3 and S4. |
Reprinted from Cell, 184, Wu, J., Lu, Z., Jiang, D., Guo, Y., Qiao, H., Zhang, Y., Zhu, T., Cai, Y., Zhang, X., Zhanghao, K., Xie, H., Yan, T., Zhang, G., Li, X., Jiang, Z., Lin, X., Fang, L., Zhou, B., Xi, P., Fan, J., Yu, L., Dai, Q., Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale, 3318-3332.e17, Copyright (2021) with permission from Elsevier. Full text @ Cell