Fig. 3
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- ZDB-FIG-230129-3
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- Wu et al., 2021 - Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale
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Figure 3. Evaluations of tiled aberration corrections with DAO (A) Orthogonal MIPs of a 100-?m-thick Thy1-YFP brain slice by SDCM, WFM, and DAOSLIMIT without aberrations. The yellow dashed lines indicate the region for xz projection. (B) Orthogonal MIPs of the same area after introducing a specific aberration obtained by WFM and DAOSLIMIT with and without DAO. Groundtruth aberration is shown in the inset of WFM result, while estimated aberration by DAO is shown in the DAOSLIMIT result. (C and D) Cross-section profiles marked by the arrows in (A) and (B), respectively. (E) Color-coded MIPs of the 3D mitochondrial dynamics by DAOSLIMIT with no corrections, system corrections only, and full DAO corrections. (F) Estimated average aberrations at different areas in (E). (G) Reconstructed slice of the region in (E). (H) Orthogonal MIPs from 2-?m-thick slabs of the region in (E), with cross-section profiles. (I) Reconstructed slice of (G) with the correction wavefront estimated in (H). (J) Reconstructed orthogonal MIPs of (H) with the correction wavefront estimated in (G). (K) Color-coded MIPs of the region in E to show a mitochondrial fusion process. (L) Histograms of the tracking length and average speed with the same tracking algorithm applied to the 2D video with DAO (n = 954 mitochondria tracked), the 3D video without DAO (n = 1,642 mitochondria tracked), and the 3D video with DAO (n = 2,540 mitochondria tracked). The dashed lines represent the mean values. Scale bars: 20 ?m (A and B), 10 ?m (E, G, and I), 2 ?m (H and J), and 1 ?m (K). See also Figure S5. |
Reprinted from Cell, 184, Wu, J., Lu, Z., Jiang, D., Guo, Y., Qiao, H., Zhang, Y., Zhu, T., Cai, Y., Zhang, X., Zhanghao, K., Xie, H., Yan, T., Zhang, G., Li, X., Jiang, Z., Lin, X., Fang, L., Zhou, B., Xi, P., Fan, J., Yu, L., Dai, Q., Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale, 3318-3332.e17, Copyright (2021) with permission from Elsevier. Full text @ Cell