Figure 3

The evaluation of the morphological defects in lasp1 MO morphants. (A) Representative images of the phenotypes of zebrafish embryos injected with 10 µM control morpholino (STD MO) or 10 µM lasp1 MO and photographed at 48 hpf. Morphants exhibited different morphological defects, including the severe phenotype with underdeveloped body and heart edema (a); undeveloped eyes and reduced tail size (b); and the mild phenotype with reduced head size, hypopigmentation and thick yolk extension (c). (B) No differences were observed between embryos injected with lasp1-MO alone and those injected with the combination of lasp1 MO and p53 MO. Scale bars: 200 µm. (C) Analysis of morphological phenotypes in lasp1 MO morphants revealed developmental defects. Most of the 10 µM and 100 µM lasp1 1 MO morphants revealed mild or severe phenotypes; the ST MO and the 1 µM lasp1 MO morphants mainly showed the wild-type phenotype. The number under each bar is the total number of embryos examined under each experimental condition. Three biological replicates were used for each assay.