Figure 6

Co-injection of lasp1 MO and LASP1 mRNA rescues the morphological defects of morphants. (A) Representative Western blotting and relative quantifications of Lasp1 protein levels in 48 hpf embryos injected with STD MO, 10 µM lasp1 MO or co-injected with 10 µM lasp1 MO and LASP1 mRNA (50 ng). GAPDH was used as an internal control. The histograms represent the mean IOD (integrated optical density) values in percentages; the bars are SDs. (B) Analysis of phenotypes and corresponding representative images of the zebrafish embryos injected with 10 µM control morpholino (STD MO), 10 µM lasp1 MO or co-injected with 10 µM lasp1 MO and human LASP1 mRNA (50 ng) and photographed at 48 hpf. The number under each bar is the total number of embryos examined under each experimental condition. Three biological replicates were used for each assay.