FIGURE

Figure 5

ID

ZDB-FIG-230122-68

Publication

Shi et al., 2023 - Single-cell transcriptomic profiling of the zebrafish inner ear reveals molecularly distinct hair cell and supporting cell subtypes

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Figure 5

Distinct markers separate macula and crista supporting cells.

(A) Feature plots showing expression of macula supporting cell marker tectb and crista supporting cell marker zpld1a. (B–D) HCR in situ hybridization in myo6b:GFP transgenic animals. Each set of images shown represents a projection of one z-stack split into cristae (lateral) and macula (medial) slices. Lateral line neuromasts positioned over the ear are visible in lateral slices. Expression pattern for (B) the pan-supporting cell marker lfng, (C) macula-specific marker tectb, and (D) crista-specific marker zpld1a in 5 dpf myo6b:GFP fish. Each set of images shown represents a projection of one z-stack split into cristae (lateral) and macula (medial) slices. ac: anterior crista, lc: lateral crista, nm: neuromast, pc: posterior crista, u: utricle, s: saccule. Scale bars = 20 μm.

Expression Data

Genes:	lfng tectb zpld1a
Fish:	w186Tg
Anatomical Terms:	anterior crista auditory epithelial support cell lateral crista macula posterior crista
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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