(A) Feature plots showing differential expression of cabp1b and cabp2b among crista and macula hair cells. (B–D) HCR in situ projections of individual sensory patches from 5 dpf myo6b:GFP fish showing differential spatial expression patterns of cabp1b and cabp2b. (B) cabp1b is expressed at the ends of the cristae, while cabp2b is expressed centrally. Anterior crista is shown. (C) In the utricle, cabp1b is expressed medially and cabp2b is expressed laterally. (D) In the saccule, cabp1b is expressed in peripheral cells at the dorsal and ventral edges of the organ. cabp2b is expressed centrally. Scale bars for HCR images = 10 μm. (E) Cartoon illustrations of the zebrafish utricle, saccule, and lagena, and the expression patterns of cabp1b (yellow) and cabp2b (magenta) within each sensory patch. (F–H) Wholemount RNAScope confocal images of adult inner ear organs showing peripheral expression pattern of cabp1b (n=3) in the adult zebrafish (F) utricle, (G) saccule, and (H) lagena. (I–K) Whole-mount RNAScope confocal images showing central expression pattern of cabp2b (n=4) in the adult zebrafish (I) utricle, (J) saccule, and (K) lagena. Scale bars for RNAScope images = 25 μm.
