Fig 1
Analysis of survival ratios in activin β subunit mutants.
(A) Ratios of different genotypes in the offspring of heterozygotes (+/- x +/-) at adult stage. (B) Ratios of different genotypes in the offspring of inhbaa+/- x inhbaa+/- from 1 to 22 dpf. Sixty fish were sampled at each time point (n = 60). (C) Ratios of different genotypes in the offspring of inhbaa+/-;inhbab-/- x inhbaa-/-;inhbab+/- from 1 to 22 dpf. Sixty fish were sampled at each time point (n = 60). (D) Kaplan–Meier plot for activin βA single and double mutants (50 to 165 dpf; n = 46–54). (E) Ratios of different genotypes in the offspring of inhbaa+/-;inhbab-/-;inhbb+/- (♀) x inhbaa-/-;inhbab+/-;inhbb-/- (♂) from 5 to 20 dpf. The number of fish examined is shown on top of each column. (F) The larvae of control (inhbaa+/-;inhbab+/-), βA double mutant (inhbaa-/-;inhbab-/-) and βA/B triple mutant (inhbaa-/-;inhbab-/-;inhbb-/-) from 5 to 15 dpf. No triple mutant individuals could survive to 15 dpf. (G) Offspring of inhbaa-/-;inhbab-/- (♀) x inhbaa-/-;inhbab-/- (♂) and inhbb-/- (♀) x inhbb-/- (♂) at 72 hpf. The asterisk indicates significant deviation from expected ratios by Chi-square analysis (p<0.05). |