FIGURE 7
Co-localization of Loxhd1b and BDNF. (A) In situ hybridization at 48–96 hpf showing that BDNF is expressed around the inner ear and olfactory pore of zebrafish, suggesting the effect of BDNF on hearing development. The red arrow indicates the olfactory pore of the zebrafish, and the red dotted circle indicates the inner ear of the zebrafish. (B) Immunofluorescence staining of Loxhd1b and BDNF in HEI-OC1 cells. The nuclei are stained blue by DAPI. Scale bar = 35 μm. (C) Representative images of inner ear hair cells in cochleae from C57 mice of 7 days old. The inner ear hair cells are labeled with Myosin VII (blue). Scale bar = 55 μm. (D) Representative images of inner ear hair cells in cochleae from p57 mice of 7 days old. Phalloidin is used to label the inner ear hair cells (green). Scale bar = 55 μm. |
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Stage Range: | Long-pec to Day 4 |