FIGURE

Figure 6

ID

ZDB-FIG-221211-26

Publication

Forte-Gomez et al., 2022 - Structure, evolution and expression of zebrafish cartilage oligomeric matrix protein (COMP, TSP5). CRISPR-Cas mutants show a dominant phenotype in myosepta

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Figure 6

Mutant ΔI258D259Comp is expressed in zebrafish. (A) location of the CRISPR-Cas introduced deletion ΔI258D259 (Δ ID) in the first TSP type-3 repeat (red bar below) and comparison with mutations in chondrodysplasia patients (46) (above in red). (B) immunoblot analysis using an affinity-purified rabbit antiserum specific for zebrafish Comp (left) of direct extracts from 2-month-old wild type (wt), and heterozygous (het) or homozygous (hom) ΔI258D259Comp (Δ ID) zebrafish that were submitted to electrophoresis on 4-10% gradient SDS-polyacrylamide gels under non-reducing conditions. Ponceau staining shows equal loading (right). (C) whole mount immunofluorescence (green) staining of 72 hpf wild type (wt) (n=6), or heterozygous (het) (n=10) and homozygous (hom) (n=4) ΔI258D259Comp zebrafish mutants were performed using an affinity-purified guinea pig antiserum specific for zebrafish Comp and detected patchy Comp deposition in myosepta of heterozygous and homozygous mutants. Bar: 150 µm.

Expression Data

Gene:	comp
Antibodies:	Ab1-comp Ab2-comp
Fish:	KOLN comp^{zf3751/zf3751} (KOLN) comp^zf3751/+ (KOLN)
Anatomical Terms:	myoseptum whole organism
Stage Range:	Protruding-mouth to Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	comp^{zf3751/zf3751} (KOLN) comp^zf3751/+ (KOLN)
Observed In:	myoseptum whole organism
Stage Range:	Protruding-mouth to Days 45-89

Phenotype Detail

Acknowledgments

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