FIGURE

Figure 4

ID

ZDB-FIG-221211-24

Publication

Forte-Gomez et al., 2022 - Structure, evolution and expression of zebrafish cartilage oligomeric matrix protein (COMP, TSP5). CRISPR-Cas mutants show a dominant phenotype in myosepta

Other Figures

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Figure 4

Immunoblot analysis of recombinant zebrafish Comp and Comp in extracts from zebrafish embryos. (A) Recombinant zebrafish COMP and (B) sequentially extracted proteins from whole 5 dpf zebrafish were separated in 0.5% (w/v) agarose/3% (w/v) polyacrylamide composite gels under non-reducing conditions and detected with guinea pig polyclonal antibodies specific for zebrafish Comp. Buffer I, TBS; buffer II, 50 mM Tris, pH 7.4, 10 mM EDTA, and 1M NaCl, buffer III, 50 mM Tris, pH 7.4, 10 mM EDTA and 8M urea. Recombinant rat COMP was used as a marker and the position of the pentamer is indicated.

Expression Data

Gene:	comp
Antibody:	Ab1-comp
Fish:	KOLN
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)