ZFIN Image: Forte-Gomez et al., 2022, Figure 6

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Figure Caption

Figure 6

Mutant ΔI258D259Comp is expressed in zebrafish. (A) location of the CRISPR-Cas introduced deletion ΔI258D259 (Δ ID) in the first TSP type-3 repeat (red bar below) and comparison with mutations in chondrodysplasia patients (46) (above in red). (B) immunoblot analysis using an affinity-purified rabbit antiserum specific for zebrafish Comp (left) of direct extracts from 2-month-old wild type (wt), and heterozygous (het) or homozygous (hom) ΔI258D259Comp (Δ ID) zebrafish that were submitted to electrophoresis on 4-10% gradient SDS-polyacrylamide gels under non-reducing conditions. Ponceau staining shows equal loading (right). (C) whole mount immunofluorescence (green) staining of 72 hpf wild type (wt) (n=6), or heterozygous (het) (n=10) and homozygous (hom) (n=4) ΔI258D259Comp zebrafish mutants were performed using an affinity-purified guinea pig antiserum specific for zebrafish Comp and detected patchy Comp deposition in myosepta of heterozygous and homozygous mutants. Bar: 150 µm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)