IMAGE

Figure 4

ID

ZDB-IMAGE-221211-29

Genes

comp

Antibodies

Ab1-comp

Publication

Forte-Gomez et al., 2022 - Structure, evolution and expression of zebrafish cartilage oligomeric matrix protein (COMP, TSP5). CRISPR-Cas mutants show a dominant phenotype in myosepta

All Figures

Figures for Forte-Gomez et al., 2022

Image

Figure Caption

Figure 4

Immunoblot analysis of recombinant zebrafish Comp and Comp in extracts from zebrafish embryos. (A) Recombinant zebrafish COMP and (B) sequentially extracted proteins from whole 5 dpf zebrafish were separated in 0.5% (w/v) agarose/3% (w/v) polyacrylamide composite gels under non-reducing conditions and detected with guinea pig polyclonal antibodies specific for zebrafish Comp. Buffer I, TBS; buffer II, 50 mM Tris, pH 7.4, 10 mM EDTA, and 1M NaCl, buffer III, 50 mM Tris, pH 7.4, 10 mM EDTA and 8M urea. Recombinant rat COMP was used as a marker and the position of the pentamer is indicated.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)