Fig. 2

(A) Expression patterns of sws1 and arr3b (SWS1 and SWS2 cones) in 5-dpf larval eyes of the foxq2 mut (ja74) examined by in situ HCR. Magnified view (a box surrounded with white lines) is indicated in the right side of each panel. Scale bars, 20 μm. (B) Fluorescent images of the flat-mounted retinas prepared from the adult WT, the foxq2 mut (ja74), and Tg(-3.5opn1sw2:EGFP)^kj11Tg (sws2:egfp), where EGFP is expressed in SWS2 cones (green). The retinas were immunostained with zpr1 antibody (arr3a, red) and also stained with DRAQ5 to highlight cell nuclei (blue). V, SWS1 cone; B, SWS2 cone; G, RH2 cone; R, LWS cone. Scale bar, 10 μm. See also fig. S3B. (C) Expression patterns of phototransduction genes in the flat-mounted retinas examined by in situ HCR. See also fig. S3C. (D) Left: Fluorescent images in retinal cryosections from the adult fish labeled for terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL) (red). The cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 50 μm. Right: Quantification of TUNEL-positive cells in the central and peripheral retina. The numbers of TUNEL-positive cells were counted for each cryosection and averaged (means ± SEM, n = 80 for WT, n = 63 for the foxq2 mut; *P < 0.05, Student’s t test). See also fig. S3D. (E) Expression patterns of sws2, rh2-1, and rh2-2 in 5-dpf larval eyes of WT and foxq2 mut (ja74) examined by in situ HCR. The number of opsin gene–positive cells in the central region of the retina is indicated in a bar graph. The number in the upper-right corner for each panel represents the unique identity of the eye. Data are represented by means ± SEM (n = 3). Scale bar, 50 μm. See also fig. S3E.