FIGURE

Fig. 5

ID

ZDB-FIG-221028-20

Publication

Cacialli et al., 2022 - Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebrates

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Fig. 5

Slco2b1 is necessary to import PGH2 into ECs

A
Fluorescence imaging of the CHT of double transgenic cd45:CFP‐NTR/runx1:mcherry embryos injected with control‐ or slco2b1‐morpholinos, after treatment with DMSO.
B
Fluorescence imaging of the CHT of double transgenic cd45:CFP‐NTR/runx1:mcherry embryos injected with control‐ or slco2b1‐morpholinos, after treatment with DMSO+MTZ_.
C
Fluorescence imaging of the CHT of double transgenic cd45:CFP‐NTR/runx1:mcherry embryos injected with control‐ or slco2b1‐morpholinos, after treatment with DMSO+MTZ + PGH2.
D
Quantification of runx1:mcherry‐positive cells. Each dot represents the number of red cells for each embryo (biological replicates). Each experiment has been repeated three independent times.
E
Quantification of cd45:CFP‐positive cells_. Each dot represents the number of blue cells for each embryo (biological replicates). Each experiment has been repeated three independent times. Data information for (D) and (E): Statistical analysis was completed using one‐way ANOVA and multiple comparison tests. ****P < 0.0001. Center values denote the mean, and error bars denote s.e.m.

Scale bar is 200 μm (A–C).

Source data are available online for this figure.

Expression Data

Genes:	CFP mCherry
Fish:	cz2010Tg/cz2010Tg; ug108Tg/ug108Tg cz2010Tg/cz2010Tg; ug108Tg/ug108Tg + MO2-slco2b1
Conditions:	chemical treatment by environment: dimethyl sulfoxide chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole
Knockdown Reagent:	MO2-slco2b1
Anatomical Terms:	hematopoietic multipotent progenitor cell myeloid cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cz2010Tg/cz2010Tg; ug108Tg/ug108Tg cz2010Tg/cz2010Tg; ug108Tg/ug108Tg + MO2-slco2b1
Conditions:	chemical treatment by environment: dimethyl sulfoxide chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole
Knockdown Reagent:	MO2-slco2b1
Observed In:	hematopoietic multipotent progenitor cell myeloid cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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