FIGURE

Fig. 4

ID
ZDB-FIG-221028-19
Publication
Cacialli et al., 2022 - Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebrates
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Fig. 4

The defect in HSPCs proliferation in slco2b1‐deficient embryos can be rescued by PGE2 treatment

  • A
    Anti‐GFP and pH3 immunostainings of either controls (Ctrl‐Mo) or slco2b1‐morphants (slco2b1‐MO) cmyb:GFP embryos.
  • B
    Quantification of the number of the pH3+ HSCs in controls or slco2b1‐morphants. Center values denote the mean, and error values denote s.e.m, statistical analysis was completed using an unpaired two‐tailed t‐test. ***P < 0.001.
  • C
    Experimental outline to measure PGE2 level by ELISA kit in control and slco2b1‐MO at 60 hpf.
  • D
    Quantification of PGE2 concentration in control‐ and slco2b1‐morphants. The statistical analysis was completed using an unpaired two‐tailed t‐test *P < 0.01. Center values denote the mean, and error bars denote s.e.m.
  • E
    Fluorescence imaging in the CHT of cmyb:GFP embryos injected with control‐ and slco2b1‐MOs and treated with PGE2.
  • F
    Quantification of GFP‐positive cells. Statistical analysis: one‐way ANOVA, multiple comparison, *P < 0.01; ****P < 0.0001. Center values denote the mean, and error bars denote s.e.m. Data information: Scale bar is 50 μm (A); 200 μm (E).

Source data are available online for this figure.

Expression Data
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Pec-fin

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Pec-fin

Phenotype Detail
Acknowledgments
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