Fig. 2

Fig. 2. Diverse zfIMPDHs cytoophidium-forming properties in vitro. (A?C) zfIMPDH isoforms were cloned with N-terminal tags and expressed in HeLa cells. Immunofluorescence probing on transfected HeLa cells under conditions with or without treatment of MPA (100 ??M for 2 ?h). Myc-tag, HA-tag and Flag-tag are shown in red and antibody against IMPDH is shown in green. Cytoophidia formed by zfIMPDH are indicated by arrows in B and C. The proportion of cells with cytoophidia and the number of cells were counted (n) is shown below each panel. (D) HEp-2 ?cells expressing zfIMPDH1a were treated with DON (100 ??M for 2 ?h) before fixation. Cytoophidia and clumps that contain zfIMPDH1a are indicated by arrows and arrowheads, respectively. Scale bars ?= ?10 ??m. (E) Bar graph showing the quantification of the proportion of zfIMPDH expressing HEp-2 ?cells with cytoophidia or clumps. Only transfected cells were counted and the number of cells counted in each group is shown as (n). (Error bars ?= ?S.E.M. ?p ?< ?0.05; ??p ?< ?0.01; ???p ?< ?0.001, proportions were compared by t-test with the respective isoform without treatment.) In all groups with MPA treatment, endogenous cytoophidia in non-transfected cells are labeled by the ?-IMPDH antibody.