FIGURE

Fig. 4

ID

ZDB-FIG-220926-41

Publication

Yao et al., 2022 - Skeletal Class III Malocclusion Is Associated with ADAMTS2 Variants and Reduced Expression in a Familial Case

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Fig. 4

ADAMTS2 was involved in several important pathological pathways based on gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. (A) The top 10 GO terms by gene ratio in the biological process terms and cellular component terms and molecular function terms in the enrichment analysis. (B) The top 20 most significantly enriched pathways by p-value with the genes co-expressed with ADAMTS2 in the KEGG analysis. (C) Egfr was co-expressed with Adamts2 during mouse embryonic development of upper and lower jaw (E10.5–E14.5). (D) qRT-PCR analysis of the expression of egfr in the adamts2-knockdown zebrafish embryos at 72 hpf. (E) qRT-PCR and Western blot analysis of the ADAMTS2 knockdown efficiency in hMSCs. (F) Western blot analysis of the expression of pathway key genes and osteogenic indicators in hMSCs. Bars represent group means. ** p < 0.01, *** p < 0.001. n = 4 independent replicates for all experiments.

Expression Data

Genes:	adamts2a egfra
Fish:	TU TU + CRISPR1-adamts2a + CRISPR2-adamts2a + CRISPR3-adamts2a + CRISPR4-adamts2a
Knockdown Reagents:	CRISPR1-adamts2a CRISPR2-adamts2a CRISPR3-adamts2a CRISPR4-adamts2a
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + CRISPR1-adamts2a + CRISPR2-adamts2a + CRISPR3-adamts2a + CRISPR4-adamts2a
Knockdown Reagents:	CRISPR1-adamts2a CRISPR2-adamts2a CRISPR3-adamts2a CRISPR4-adamts2a
Observed In:	whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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