FIGURE 3

Hair cell toxicity of lopinavir and ritonavir combinations. DAPI-labeled fish were treated for 24 h with 10 and/or 50 μM of lopinavir (L), ritonavir (R), or both drugs. (A) Representative images of each drug combination; representative images of each drug alone are shown in Figure 1. Scale bar in the upper left panel = 10 μm and applies to all images. (B) There was a significant effect of drug treatment on hair cell number [one-way ANOVA, F₍₈,₁₁₂₎ = 42.68, p < 0.0001]. Asterisks indicate significant differences from vehicle controls (****p < 0.0001). There were no differences between fish treated with different combinations of both drugs (10/10, 10/50, 50/10, or 50/50). Statistical analysis for all pairwise comparisons is shown in Supplementary Table 1. Note that the 10 and 50 μM lopinavir data (without ritonavir) are also presented in Figure 2. Data are represented as mean ± 1 s.d. and black dots represent individual fish (N = 9–22/treatment).